Left: Images of fluorescent particles that are above, at and below (top to bottom) the vertical position of best focus of a microscope. Calibrating the effects of lens aberrations on the apparent ...

(Nanowerk Spotlight) The drawbacks of existing measures for calibrating scanning probe microscopes (SPMs) based on various diffraction gratings and periodic ...

Current calibration methods rely on artificially constructed DNA structures or specific cellular features, each with significant drawbacks. DNA-based rulers require complex chemical synthesis and only ...

New work enables optical microscopes to measure these nanometer-scale details with a new level of accuracy. Over the last two decades, scientists have discovered that the optical microscope can be ...