Cancer cells release their DNA into the patient's bodily fluids and cancer-specific signatures can be recognized in the circulating DNA. The aberrant methylation of CpG-rich regions in gene promoter ...
First, the BAC clone DNA is denatured into single stranded templates. Each of the three DOP-PCR primers used in the next step is flanked by known 5' and 3' anchor sequences with six random nucleotides ...
The primary bottleneck in this procedure is the inability to culture forensically relevant pathogens under laboratory conditions; moreover, nucleic acids may be low quality or damaged, rendering them ...
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