The MTS assay is often described as a 'one-step' MTT assay, which offers the convenience of adding the reagent straight to the cell culture without the intermittent steps required in the MTT assay.

The MTT assay is a cornerstone method in cell biology for evaluating cell viability and proliferation. It is especially valuable in drug discovery, toxicology, and cancer research.

We investigated the applicability and limitations of the MTT assay in representing treatment toxicity, cell viability, and metabolic activity.

Dec 19, 2025 · The MTT assay, abbreviated “ 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ” assay, is a colorimetric assay frequently used to evaluate cell viability and proliferation.

The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity.

The MTT assay is a widely used laboratory technique in cell biology research. It provides a simple and reliable way to assess the health and growth of cells in a controlled environment.

Jun 9, 2025 · The MTT assay is used to study apoptosis by measuring the decrease in cell viability upon induction of apoptosis. A reduction in the number of viable cells indicates the occurrence of apoptosis.

CyQUANT MTT and XTT function as cell health indicators by using the cellular redox potential of live cells to convert a tetrazolium-based salt reagent, MTT or XTT, to a brightly colored formazan product.

MTT is a tetrazolium salt that is turned into a purple formazan product after reduction by mitochondrial enzymes that are only present in metabolically active live cells. The amount of formazan product …

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications. Use the Chromatogram Search to identify …