A549 cell - Alchetron, The Free Social Encyclopedia
A549 cell - Alchetron, The Free Social Encyclopedia
Dec 2, 2017
alchetron.com
Flow Cytometry Protocol for Cell Surface Markers
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Below, you can see a human lung epithelial cell line A549, stained with MitoTracker™ Orange (mitochondria) and SIR-DNA (nuclei). A time lapse of 2.5 hours was acquired using a 40x magnification with a numerical aperture of 0.95. This acquisition is a good example of how ZEISS Celldiscoverer 7 and LSM 900 seamlessly combine two imaging modes. Fluorescent channels were acquired in confocal mode using highly sensitive GaAsP detectors while the Phase Gradient Contrast is acquired with a camera. Samp
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Below, you can see a human lung epithelial cell line A549, stained wi…
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